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Animal Behaviour - Introduction to Psychopharmacology

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Sedatives – Drugs that depress the central nervous system, reducing anxiety and inducing calmness or sleep (e.g., benzodiazepines, barbiturates, azapirones).

What are the major drug psychoactive classes?

- Sedatives-

Benzodiazepines

Barbituates

Azapirones

- Antidepressives and anxiety -

TCA

SSRI

MAO inhibitors

- Neuroleptic/Antipyschotics eg. ACP, chlorpromazine, haloperidol

- epilepsy and mania

Anti-convulsants

-Sedative

Antihistamines

B Blockers

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Key Terms

Term
Definition

What are the major drug psychoactive classes?

- Sedatives-

Benzodiazepines

Barbituates

Azapirones

- Antidepressives and anxiety -

TCA

SSRI

MAO inhibito...

When may B blockers be used?

Combat the somatic signs of aniety which could be detected by body -> feelings of fear

Which drugs are used as anti depressive in humans/ anxiety in animals? Which other condition may one of these be useful for?

TCAs

SSRIs

MAO Inhibitiors

- may also be useful in age related cog decline (dementia)

Give examples of drugs acting at the cell membrane

Adrenaline

Give examples of drugs acting at a nuclear receptor and impacting protein synthesis

Sex hormones, GH (Steroids)

Give examples of drugs acting on intracellular enzymes

MAO inhibtiors

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TermDefinition

What are the major drug psychoactive classes?

- Sedatives-

Benzodiazepines

Barbituates

Azapirones

- Antidepressives and anxiety -

TCA

SSRI

MAO inhibitors

- Neuroleptic/Antipyschotics eg. ACP, chlorpromazine, haloperidol

- epilepsy and mania

Anti-convulsants

-Sedative

Antihistamines

B Blockers

When may B blockers be used?

Combat the somatic signs of aniety which could be detected by body -> feelings of fear

Which drugs are used as anti depressive in humans/ anxiety in animals? Which other condition may one of these be useful for?

TCAs

SSRIs

MAO Inhibitiors

- may also be useful in age related cog decline (dementia)

Give examples of drugs acting at the cell membrane

Adrenaline

Give examples of drugs acting at a nuclear receptor and impacting protein synthesis

Sex hormones, GH (Steroids)

Give examples of drugs acting on intracellular enzymes

MAO inhibtiors

Define drug

A substance that induces a biological response

Define receptor

A cellular drug target

Define pharmacokinetics and pharmacodynamics

Kinetics - What the body does to the drug

Dynamics - What the drug does to the body

Define affinity, efficacy and specificity

Affinity - ability to interact with a receptor

Efficacy - ability to induce a biological response

Specificity - NO drug is truly specific - as the conc^ will exert other actions

How do antagonists and agonists differ in functionality?

Agonist - affinity and efficacy

Antagonist - has affinity but NEVER efficacy (always =0)

What are the different types of agonist?

Full

Partial

Inverse

What is digoxin used for?

Congestive heart failure - +inoptrope

Define threshold concentration

Lowest dose to give an effect

Define max dose

Maximum dose before side effects begin to majorly increase

Give examples of a partial agonist

Buspirone - 5-HT modulator used for feline urinary issues, separation anxiety and feather pecking

Buprenorphine - partial opioid agonist -> less risk of reparatory depression associated with analgesia and harder to OD

What is the outcome of a partial agonist dependant upon?

The [endogenous agonist]

- if ^ -> antagonism

- if v -> agonism

Therefore acts as a MODULATOR of neurotransmission

What are the 6 types of antagonism?

Competitive (reverisble)

Competitive (Irreversible) - usually enzymes (enzyme “suicide”)

Non-competitive - inhibits the way enzymes function

Physiological

Pharmacokinetic

Chemical

Give a use of an antagonist

Dexometodine -> Aneasthesia

Competitive antagonist shifts concentration of active anaesthetic and stops it from working, thus reversing effects of anaesthesia

(Shifts dose response curve to the right)

How does non-competitive antagonism affect the shape of the dose-response curve?

MAY shift curve -> right

Decreases gradient of slope

Decreases maximum possible response

What are the two types of receptor adaptation that occur in response to drug use?

Short term - desensitisation

-> rapid

uncoupling from effector mechanism eg. G protein

internalisation of receptor

inactivation of ion channels

temporary efect

rapidly reversed

Long term - down-regulation

-> slow

changes in protein expression levels

adaptive response to long term agonist activation

secondary to other mechanisms eg. chronic stress

influenced by multiple factors

slow to reverse

What phenomena may receptor adaptation underlie?

Antidepressants

Antipyschotics

Mood stabilising drugs

Tolerance

Side effect profiles

-> May also be the therapeutic action of the drug!!

Give an example of receptor adaptation?

Exogenous steroid use -> endogenous steroid production by negative feedback (can occur with any long term medication)

Outline the different aspects of pharmacokinetics

What the body does to the drug

Administered->

Absorption->

Systemic blood circulation->

Distribution->

Tissues ->

Elimination (metabolised or excreted)

Which factors can affect absorption?

Route, frequency of dosing, half life

Which factors can affect distribution?

Plasma protein binding, lipid solubility, rate of blood flow

Which factors can affect elimination?

Half life, frequency of dosing, disease

Why do heavy metal and DDT poisoning occur?

The elimination pathway of their pharmacokinetics is not functional!

When do drug interactions occur?

Build up of substrate and insufficient enzyme -> interactions

eg. dysfunctional P450 hepatic enzyme system which can occur genetically

What must be remembered about psychiatric drugs?

Many have active metabolites which can build up

Most drug interactions are more for academic or marketing interest than clinically relevant

Some have low Tis eg. TCAs like clomipramine

Give 5 pharmacological targets for neurotransmission

Post-synaptic receptors eg. buspirone (5-HT1a), diazepam (GABAa)

Auto/hetero receptors - negative feedback from the synapse stimulated by tonic NT levels eg. mirtazipine (a2-adrenoceptor) appetite stimulus, antidepressant and sleep disorder drug

Enzymes eg. selegeline (MAOb inhibitor) neuroprotective action

Reuptake transporters eg. clomipramine (TCA), fluvoxetine (SSRI)

Ion channels

What actions do successful/effective drugs tend to have?

Broad effects at multiple sites (this -> ^risk of side effect though)

Give a psychoactive drug with a low TI

Clomipramine (TCA) for separation anxiety

cardiotoxicity with arrythmias

no antidote

delayed onset

Give the 6 drug classes used in the treatment of anxiety

1- Benzidiazepines - amnesic, anxiolytic, hypnotic, NOT LONGTERM, tolerance and dependence)

2- Barbituates - anticonvulsant, anaesthetic, v TI (Petabarbitone = euthanasia) CNS depressant

3- Azapirones - antipyschotic, motor sedative (voluntary movement only, reflexes still work) DA antag. eg. Stresnil (pig aggression,) Fluoperidol SHORT TERM behaviour control

4-Buspirone - nonsedating anxiolytic 5-HT1a partial agonist

5- Antihistmines (sedative) eg. piritone

6- B-blockers - treat somatic signs of anxiety

What are the actions of ACP

Sedative but REFLEXES ARE NOT AFFECTED - give analgesia too

What are the 3 GABA receptors?

GABAa - ligand gated ion channel, pentameric, Cl- channel

GABAb - G-protein coupled receptor, activting K+ channels

GABAc - ligand gated ion channel Cl-

What is the mechanism of action of benzodiazepines?

Enhance response of GABA - facilitate opening of GABA activated Cl- channel

Bind to regulatory site

^affinity for GABA

^frequency of opening

Do not affect conductance or mean opening time

potentiates GABA effectiveness essentially

What ar the effects and uses of Benzodiazepines?

-v anxiety and aggression

sedation, hypotic

reduced muscle tone and coordination

anticonvulsant

retrograde amnesia

paradoxical increase in irritability and aggression (DISINHIBITION)

unwanted effects

acute overdose

tolerance and dependance

side effects during therapy eg. sedation when wanted as an anxiolytic

What may underlie anxiety neurologically?

Distribution of GABA receptors (potentially)

Serotonin

-Dense 5-HT input to locus coerulus and amygdala which spark a fear response before visual recognition of the stimulus itself

-Low 5-HT activity may -> dysregulation of other neurotransmitters eg. NA

-Reciprocal interactions eg. medial PFC connected wit hamygdala, allowing regulation of affect and modulation of autonomic and neuroendocrine function allowing cognitive control of anxiety response

-STEIN'S theory suggests ^5-HT -> anxiety disorders

What is the main use of Azaperones?

Anxiolytic and aggression

What is the pharmacological activity of azaperones like?

Chemical straightjacket! - Multiple actions at multiple receptors

Give an example of an azaperone

Buspirone - partial 5-HT1a agonist

How does endogenous ligand concentration affect the actions of partial agonists?

If endogenous ligand conc is high, acts as an antagonist by competing for receptors (thus moves curve -> right)

If endogenous ligand conc is low, acts as an agonist but can only produce a sub maximal response

Following the onset of a shock or fear response, what should happen?

NA and 5-HT increase, then are Downregulated by prefrontal cortex

How is histamine related to the brain?

Present in low levels in the CNS

hypothalamc neurones with widespread axonal innervations

H1 in cortex and reticular activating system aontribute to arousal and wakefulness (therefore H1 antags are sedative)

Also involved in emesis, regulation of food and water intake, thermoreg

What pathological state occurs when the serotonin system fails to function correctly?

Depression

Which are the monoamine NTs?

Serotonin (IMPULSE,) Noradrenaline (VIGILANCE,) Dopamine (DRIVE) - interactions as outlined by Healy and MacMonagle 1997

implicated in the control of mood, emotion and behavioural responses

regulate and are influenced by endocrine systems

Which part of the neurone does buspirone act on?

The cell body (usually in the brainstem) Downregulates 5-HT system

Why is dopamine not usually targeted for drug use in antidepressant field?

Link with cocaine/amphetamine

Give 7 drugs used in the treatment of affective disorders (OCD, depression, anxiety disorders)

TCAs eg. clomipramine, amytryptaline

SSRIs eg. Fluoxetine, paroxetine

5HT and NA reuptake inhibs eg. Venlafaxine

5HT and NA repecific antagonist eg. Mirtazapine

both of these ^NA and 5HT

NA SRIs eg. Reboxetine

Mixed uptake inhibitors and antagonists eg. nefazedone

MAO inhibitors eg. Selegeline

ALL ACT TO INCREASE MONOAMINE TRANSMISSION

What does the increased [synaptic monoamine] act to do?

Activation of somatodendritic autoreceptors - v firing rate -> hippocampal neurogenesis

Receptor adaptation up/down regulation (plasticity) -> ^[synaptic] and cognitive effects

Activation of pre-synaptic auto receptors - v NT release -> Post-synaptic receptor adaptation

Remember there are multiple causes of anxiety

Which drugs are safer, TCAs or SSRIs?

SSRIs! TCAs have a sedative muscarinic effect and give you dry mouth and blurred vision

How selective are most uptake inhibitors for 5HT or NA?

Ranges from Citalopram (most selective for 5HT) to Reboxetine (most selective for NA)

VENLAFAXINE VERY GOOD DRUG EQUAL NA AND 5HT SELECTIVITY

What are antipyschotics/neuroleptics used for?

controlling aggression (scizophrenia in humans)

- chemical straight jacket

What are the two classes of antipyschotics/neuroleptics?

Typical - DA untags (Extrapyramidal, muscarinic and Motor side effects)

Treat + symptoms of schizophrenia, multiple sites of action all D2 receptors, multiple side effects (may induce depression and parkinsons or catalepsy in animals)

Atypical - DA and 5HT antags (No motor side effects, but weight gain, insomnia, sedation side effects)

More selective targeting D2 and 5HT2 Rs, reduced side effects and therapeutic profile

Outline the antiphyschotic drug D2 R blockade

mesolimbic - reduces positive symptoms

Mesocortical - Incresases negative symptoms, cognitive deficits

Nigrostriatal - induces motor side effects (parkinsons)

Tuberoinfundibular - Effects on hormone secretion (hyperPRLeamia)

High doses of what type of drug can provide chemical restraint?

Antipyschotics eg. Aceptapromazine

What is another name for Atypical antipyschotics?

2nd generation

D2 and 5HT antagonists (hypothesised improved efficacy and reduced side effects compared to typical antipyschotics but limited evidence)

Fast off theory (fast kinetics)

R5HT2a receptors expressed in cortical, hippocampal regions especially

How can the best efficacy be achieved when formulating a drug?

Multiple therapeutic mechanisms, without multiple extra mechanisms which will evoke side effects

What physiological use do anticonvulsants have?

Mood stabilising - eg. for bipolar

enhanced GABA action eg. phenobarbital

Inhibition of sodium channels eg. Carbamazepine, phenytoin

Inhibition of calcium channels eg. Ethosuximide, gabapentin

reduce electrical excitability of cell membranes through USE DEPENDANT Na channel blockade

What type of drug is Selegeline?

MAOb inhibitor

enhance DA reelase and blockade of reuptake

does not induce food interactions

Where does MAob predominate?

DA rich areas of CNS

How may MAOb be neuroprotective? (i.e. for use in dementia in dogs and cats)

Reduces oxygen free radical production (up regulates superoxide dismutase)

Delays apoptosis

sows decline, does NOT prevent