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Identification and taxonomy of bacteria

Biology11 CardsCreated 3 months ago

A comprehensive flashcard set covering the structural differences between Gram-positive and Gram-negative bacteria, the nature of endospores, major bacterial groups, and key taxonomic classifications. Ideal for microbiology students and exam review.

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Explain the different between gram positive and gram negative cell walls for bacteria

gram positive cell wall; has a thick layer of peptidoglycan however for gram -ve its small
game +ve can survive UR rays and drying out however gram -ve can’t

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Key Terms

Term
Definition

Explain the different between gram positive and gram negative cell walls for bacteria

gram positive cell wall; has a thick layer of peptidoglycan however for gram -ve its small
game +ve can survive UR rays and drying out however g...

What is an endospore

Endospore
produce by gram +ve
very resistant to drying out and uv light
spores are not permeable
in the body they are dominant until th...

List the groups of bacteria

groups of bacteria

-Mollicutes

-Cyanobacteria

List the classification of bacteria

classification of bacteria
genus; collection of different species sharing major properties
species; small group of organism which are hard to...

List the ways of identifying bacterial

List the ways of identifying bacterial
direct methods; need to increase and purity the bacteria
eg. culture
Molecular methods (PCR amplifi...

Explain culturing for identification for bacteria

culturing for identification
purpose; ID bacteria based upon appearance
permits selections and transfer of different species from a mixed cul...

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Identification and taxonomy of bacteria
TermDefinition

Explain the different between gram positive and gram negative cell walls for bacteria

gram positive cell wall; has a thick layer of peptidoglycan however for gram -ve its small
game +ve can survive UR rays and drying out however gram -ve can’t

What is an endospore

Endospore
produce by gram +ve
very resistant to drying out and uv light
spores are not permeable
in the body they are dominant until the right conditions come along

List the groups of bacteria

groups of bacteria

-Mollicutes

-Cyanobacteria

-Fusobacteria

-Proteobacteria: gram -ve

PVC group

FBC group

spirochetes

Acinetobacteria

-Firmicultes; mostly gram +ve

List the classification of bacteria

classification of bacteria
genus; collection of different species sharing major properties
species; small group of organism which are hard to distinguishing
biovar; strains of species with different biochemical and phenotypical characteristics
serovar; stain of species which differs in serology
phagetype; bacteria specific phages used (defines clones)
genotype: defines clade, determined using DNA fingerprinting methods

List the ways of identifying bacterial

List the ways of identifying bacterial
direct methods; need to increase and purity the bacteria
eg. culture
Molecular methods (PCR amplification, cloning)
or indirectly; immune response in the host

Explain culturing for identification for bacteria

culturing for identification
purpose; ID bacteria based upon appearance
permits selections and transfer of different species from a mixed culture
a single bacterial cells is put on the surface of a nutritive medium and it begins to divide

For cultures for identification of bacteria list the different types of media and incubation

different types of media and incubation

incubation;

aerobic

anaerobic

microaerobic

CO2 enhanced

media;

enrichment

selection differential

decide on temperature which is depend on host match the hosts temperature

List the types of Haemolytic patterns on Blood agar

Types of haemolytic patterns
Gamma no haemolysis
Alpha
Beta; zones of clearing where there is totally haemolysis

List the types of preliminary identification

types of preliminary identification

catalase-

oxidase-

spot indole

motility

urease

gram stain

KOH gel formation

List the testes which are used for secondary identification

secondary identification

  • biochemical tests

  • serological tests

Define the Antimicrobial susceptibility test

Antimicrobial susceptibility test;

detection of resistance to antibiotics used to treat bacteria