Biochemistry /1.8: Factors Affecting Enzyme Action

1.8: Factors Affecting Enzyme Action

Biochemistry4 CardsCreated 17 days ago

Enzyme activity is influenced by temperature, pH, enzyme concentration, and substrate concentration. These factors affect the rate of enzyme-substrate complex formation, with optimal conditions enhancing the reaction rate and extreme conditions (like high temperature or pH changes) potentially denaturing the enzyme or limiting its effectiveness.

effect of temperature

  • rise in temp increases the kinetic energy of molecules

  • more frequent collisions between enzyme and substrate

  • more ES complexes formed in a given amount of time

  • faster rate of reaction

  • however if temp rises too much then enzymes become denatured

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Key Terms

Term
Definition

effect of temperature

  • rise in temp increases the kinetic energy of molecules

  • more frequent collisions between enzyme and substrate

  • ...

effect of pH

  • change in pH alters the charges on the amino acids that make up the active site o

  • substrate and enzyme no longer have a comp...

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effect of enzyme concentration on rate of reaction

  • as long is there is an excess of substrate, an increase in enzyme concentration leads to a proportional increase in the rate of reaction

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effect of substrate concentration on rate of reaction

  • if enzyme concentration is fixed than an increase in substrate concentration will increase the rate of reaction because the available subst...

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TermDefinition

effect of temperature

  • rise in temp increases the kinetic energy of molecules

  • more frequent collisions between enzyme and substrate

  • more ES complexes formed in a given amount of time

  • faster rate of reaction

  • however if temp rises too much then enzymes become denatured

effect of pH

  • change in pH alters the charges on the amino acids that make up the active site o

  • substrate and enzyme no longer have a complementary shape so substrate cannot bind to form ES complex

effect of enzyme concentration on rate of reaction

  • as long is there is an excess of substrate, an increase in enzyme concentration leads to a proportional increase in the rate of reaction

  • however, if there are less substrate molecules than enzyme molecules then substrate concentration is the limiting factor so increasing the enzyme concentration will not increase the rate of reaction

effect of substrate concentration on rate of reaction

  • if enzyme concentration is fixed than an increase in substrate concentration will increase the rate of reaction because the available substrates for the enzymes to collide and form ES complexes with is increased

  • however, as the substrate concentration increases further the active sites of the enzymes become fully saturated and so a further increase in substrate concentration will not increase the RoR