2020 ASCP Molecular Biology Certification Exam With Answers (100 Solved Questions)

Name: 2020 ASCP Molecular Biology Certification Exam With Answers (100 Solved Questions)
Brand: Academic Documents Platform
Price: 3 USD
Availability: InStock
Author: Olivia Parker

Learn faster with 2020 ASCP Molecular Biology Certification Exam With Answers, featuring past exam questions with expert solutions.

Olivia Parker

Contributor

4.5

about 1 month ago

Preview (4 of 11)

ASCP Molecular Biology Certification Exam
10 studiers today
Science Biology Pathology
4.8 (11 reviews)
Students also viewed
Terms in this set (1434)
MB (ASCP) 2020
Teacher 402 terms
Kquan20 Preview
Molecular Diagnostics Chapter 2
Teacher 57 terms
Lolo20000000 Preview
Membrane
29 terms
Pyrimidine One carbon ring
Cytosine, Thymine, Uracil
What is the function of
mRNA?
Carries genetic info out of nucleus
Transcript translated to protein
What is the function of
tRNA?
Carries aa to ribosome
Anticodon pairs with codon on mRNA strand
What is the function of
rRNA?
part of ribosome structure
most abundant RNA
coordinated coupling of tRNA to mRNA codons
Feedback inhibition
Product of pathway is noncompetitive inhibitor
Binds to allosteric site to slow down rxn b/c too much
product
1 of 11

Endonucleases (Prok)
Restriction enzymes
Cleaves phoshpodiester bonds w/i poly-nt chain
Recognition site is palindromic sequence
Types I-V
ORI sites nt sequence where replication is initiated
Topoisomerase I
Induces ss breaks
Remove DNA supercoils during TXN and DNA
replication; for strand breakage during recombination;
for chr condensation; and to disentangle intertwined
DNA during mitosis
topoisomerase II
cuts both strands of one DNA double helix, passes
another unbroken DNA helix through it, and then
reanneals the cut strands
Gyrase (topoisomerase II) Unwinds supercoiling caused by unwinding at the rep
fork by introducing DSBs
Helicase Breaks H-bonds of double helix at the replication fork
single-strand DNA binding
proteins (SSBPs)
Binds ssDNA and prevents it from re-annealing during
TXN, replication, repair, and recombination
Telomeres Repeat sequence (TTAGGG) at the ends of chr, protect
chr from degradation
RNA polymerase
DNA dependent RNApol
Transcribes DNA template to RNA (3'-->5'; anti-
parallel)
Splicesomes
Complex of snRNPs
Removes introns from pre-mRNA and splices exons
together
Enhancers Short regions of DNA that bind proteins (TXN factors)
that enhance TXN of a gene
Reverse transcriptase enzyme that transcribes RNA to cDNA (lacks introns)
RNA --> RNA:DNA --> cDNA (dsDNA)
2 of 11

Open Reading Frame
(ORF)
sections of DNA that begin with start codons and end
with stop codons
DNA: 5' --> 3'
transcription: 3' --> 5' DNA --> RNA (promoter)
translation: 5' --> 3' mRNA
Spectrophotometer Measures amount of light absorbed
Quantitative measurement of [DNA/RNA]
At what wavelength does
DNA and RNA absorb?
260 nm
At what wavelength does
protein absorb?
280 nm
Organic isolation method
1. Lyse
2. Add phenol/ chloroform > vortex/spin
3. Transfer aqueous layer (top) to new tube
4. Add chloroform:IAA (removes phenol) > vortex/spin
5. Transfer aqueous layer to new tube
6. Add NaOAc and EtOH > vortex/spin
7. Decant
8. Resuspend
What does the incubation
step in hybridization do?
Allows formation of ds molecules
Formamide acts as a
__________ in a hybridization.
denaturing agent
Line Probe Assay (LiPA)
reverse hybridization assay using sequence-specific
oligonucleotide probes (reverse SSOP)
multi-parameter testing --> single strip
Single-Stranded
Conformational
Polymorphism Ananlysis
(SSCP)
Used for known gene, unknown mut
Mutation screening
Short PCR products form 3D conformation when
cooled --> muts have different conformation than WT
Non-denaturing PAGE, muts migrate different than WT
3 of 11

Preview Mode

100%

Study Now!

XY-Copilot AI

Unlimited Access

Secure Payment

Instant Access

24/7 Support

Document Chat

Document Details

Subject

American Society for Clinical Pathology

2020 ASCP Molecular Biology Certification Exam With Answers (100 Solved Questions)

Study Now!

Document Details

Related Documents

ASCP Hematology Phlebotomy Certification Exam With Answers (172 Solved Questions)

Company

Explore

Study Tools