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Biology IB HL - 1.2 Cell Structure

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Prokaryotes are cells that lack a true nucleus and other membrane-bound organelles.

What organelle (main) do prokaryotes lack?

Prokaryotes are organisms whose cells lack a nucleus

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Key Terms

Term
Definition

What organelle (main) do prokaryotes lack?

Prokaryotes are organisms whose cells lack a nucleus

What 2 domains are prokaryotes divided into?

archaebacteria

- eubacteria

Where are archaebacteria found?

found in extreme environments like high temperatures, salt concentrations or pH (i.e. extremophiles)

What are eubacteria?

traditional bacteria including most known pathogenic forms (e.g. E. coli, S. aureus, etc.)

What different organelles do prokaryotes have (from eukaryotes)?

nucleoid

plasmids

slime capsule

flagella

pilli

What is a nucleoid?

region of the cytoplasm where the DNA is located (DNA strand is circular and called a genophore)

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TermDefinition

What organelle (main) do prokaryotes lack?

Prokaryotes are organisms whose cells lack a nucleus

What 2 domains are prokaryotes divided into?

archaebacteria

- eubacteria

Where are archaebacteria found?

found in extreme environments like high temperatures, salt concentrations or pH (i.e. extremophiles)

What are eubacteria?

traditional bacteria including most known pathogenic forms (e.g. E. coli, S. aureus, etc.)

What different organelles do prokaryotes have (from eukaryotes)?

nucleoid

plasmids

slime capsule

flagella

pilli

What is a nucleoid?

region of the cytoplasm where the DNA is located (DNA strand is circular and called a genophore)

What are plasmids?

autonomous circular DNA molecules that may be transferred between bacteria (horizontal gene transfer)

What size is a prokaryote ribosome?

70S

How does the cell wall in a prokaryote differ from a eukaryote?

rigid outer covering made of peptidoglycan

What is a slime capsule?

a thick polysaccharide layer

What is the function of a slime capsule?

used for protection against dessication (drying out) and phagocytosis

What is a flagella?

Long, slender projections containing a motor protein that enables movement

What are pili?

Hair-like extensions

What are the function of pili?

enable adherence to surfaces (attachment pili) or mediate bacterial conjugation (sex pili)

How do prokaryotic cells reproduce?

Binary fission is a form of asexual reproduction used by prokaryotic cells

What are the 3 steps of binary fission?

The circular DNA is copied in response to a replication signal

The two DNA loops attach to the membrane

The membrane elongates and pinches off (cytokinesis), forming two cells

What do eukaryote cells have that prokaryotes don’t?

organisms whose cells contain a nucleus

Eukaryotic cells are compartmentalised by membrane-bound structures (organelles) that perform specific roles

What 4 kingdoms can eukaryotes be categorised into?

protista

fungi

plantae

animalia

What are protista?

unicellular organisms; or multicellular organisms without specialised tissue

What are fungi?

have a cell wall made of chitin and obtain nutrition via heterotrophic absorption

What are plantae?

have a cell wall made of cellulose and obtain nutrition autotrophically (via photosynthesis)

What are animalia?

no cell wall and obtain nutrition via heterotrophic ingestion

What are organelles?

Organelles are specialised sub-structures within a cell that serve a specific function

What are 3 universal organelles (pro and eukaryote)?

ribosomes

cytoskeleton

plasma membrane

What is the structure of a ribosome?

Two subunits made of RNA and protein; larger in eukaryotes (80S) than prokaryotes (70S)

What is the function of a ribosome?

Site of polypeptide synthesis (this process is called translation)

What is the structure of the cytoskeleton?

A filamentous scaffolding within the cytoplasm (fluid portion of the cytoplasm is the cytosol)

What is the function of the cytoskeleton?

Provides internal structure and mediates intracellular transport (less developed in prokaryotes)

What is the structure of the plasma membrane?

Phospholipid bilayer embedded with proteins (not an organelle per se, but a vital structure)

What is the function of the plasma membrane?

Semi-permeable and selective barrier surrounding the cell

What is the structure of the nucleus?

Double membrane structure with pores; contains an inner region called a nucleolus

What is the function of the nucleus?

Stores genetic material (DNA) as chromatin; nucleolus is site of ribosome assembly

What is the structure of the endoplasmic reticulum?

A membrane network that may be bare (smooth ER) or studded with ribosomes (rough ER)

What is the function of the ER?

Transports materials between organelles

| smooth ER = lipids ; rough ER = proteins

What is the structure of the golgi apparatus?

An assembly of vesicles and folded membranes located near the cell membrane

What is the function of the golgi apparatus?

Involved in the sorting, storing, modification and export of secretory products

What is the structure of the mitochondrion?

Double membrane structure, inner membrane highly folded into internal cristae

What is the function of the mitochondrion?

Site of aerobic respiration (ATP production)

What is the structure of the peroxisome?

Membranous sac containing a variety of catabolic enzymes

What is the function of the peroxisome?

Catalyses breakdown of toxic substances (e.g. H2O2) and other metabolites

What is the structure of the centrosome?

Microtubule organising centre (contains paired centrioles in animal cells but not plant cells)

What is the function of the centrosome?

Radiating microtubules form spindle fibres and contribute to cell division (mitosis / meiosis)

What are 3 organelles unique to plant cells?

chloroplast

vacuole (large, central and permanent)

cell wall

What is the structure of a chloroplast?

Double membrane structure with internal stacks of membranous discs (thylakoids)

What is the function of the chloroplast?

Site of photosynthesis – manufactured organic molecules are stored in various plastids

What is the structure of a vacuole?

Fluid-filled internal cavity surrounded by a membrane (tonoplast)

What is the function of a vacuole?

Maintains hydrostatic pressure (animal cells may have small, temporary vacuoles)

What is the structure of the cell wall?

External outer covering made of cellulose (not an organelle per se, but a vital structure)

What is the function of the cell wall?

Provides support and mechanical strength; prevents excess water uptake

What is the structure of the lysosome?

Membranous sacs filled with hydrolytic enzymes

What is the function of the lysosome?

Breakdown/hydrolysis of macromolecules (presence in plant cells is subject to debate)

How do electron microscopes work?

Electron microscopes use electron beams focused by electromagnets to magnify and resolve microscopic specimens

What 2 types of electron microscopes are there?

Transmission electron microscopes (TEM)

| Scanning electron microscopes (SEM)

What do TEM produce? (image type)

generate high resolution cross-sections of objects

What do SEM produce? (image type)

display enhanced depth to map the surface of objects in 3D

What are 2 key advantages of electron microscopes compared to light microscopes?

They have a much higher range of magnification (can detect smaller structures)

They have a much higher resolution (can provide clearer and more detailed images)

What is a disadvantage of an electron microscope?

Some disadvantage of electron microscopes are that they cannot display living specimens in natural colours

What is a micrograph?

A micrograph is a photo or digital image taken through a microscope to show a magnified image of a specimen

How could we guess cell function from a micrograph?

Attempts can be made to deduce cell function based on the relative abundance of various organelles

What does it suggest about the cell if it has a large number of mitochondria?

Cells with many mitochondria typically undertake energy-consuming processes (e.g. neurons, muscle cells)

What does it suggest about the cell if it has a large ER network?

Cells with extensive ER networks undertake secretory activities (e.g. plasma cells, exocrine gland cells)

What does it suggest about the cell if it has a large number of lysosomes?

Cells rich in lysosomes tend to undertake digestive processes (e.g. phagocytes)

What does it suggest about the cell if it has a large number of chloroplasts?

Cells with chloroplasts undergo photosynthesis (e.g. plant leaf tissue but not root tissue)

What 4 features of a prokaryotic cell are not found in a eukaryote?

pili

flagella (except sperm)

slime capsule

plasmid

What size are ribosomes in a prokaryote?

labelled as 70S

What is the cell wall in a prokaryote composed of?

peptidoglycan, (thicker than cell membrane)