StudyXY

Q
QuestionBiochemistry

What units are used to measure absorbance on a spectrophotometer?
4 months agoReport content

Answer

Full Solution Locked

Sign in to view the complete step-by-step solution and unlock all study resources.

Step 1
: Absorbance is a dimensionless quantity in spectrophotometry.

A=−log⁡10(II0)=εlcA = -\log_{10} \left( \frac{I}{I_0} \right) = \varepsilon lc
In spectrophotometry, absorbance is a measure of how much light a sample absorbs at a particular wavelength. The beam of light passing through the sample is attenuated according to the Beer-Lambert Law: where: - 1$ is the concentration of the absorbing species Since absorbance is defined as the logarithm of a ratio of intensities, it does not have any physical units. It is simply a number that represents the amount of light absorbed by the sample.

Final Answer

A=−log⁡10(II0)=εlcA = -\log_{10} \left( \frac{I}{I_0} \right) = \varepsilon lc

Need Help with Homework?

Stuck on a difficult problem? We've got you covered:

  • Post your question or upload an image
  • Get instant step-by-step solutions
  • Learn from our AI and community of students

Related Questions

Biochemistry
3 months ago

The Diels-Alder reaction is a concerted reaction; this means: (a) all bond making and bond breaking occurs simultaneously. (b) the reaction follows Markovnikov's rule. (c) the reaction is highly endothermic. (d) the products contain rings. (e) a mixture of endo and exo products are formed.

·
View Answer
Biochemistry
3 months ago

"Which of the following best describes a disaccharide? It is two monosaccharides joined together. It is a group of monosaccharide pairs. It is two trisaccharides joined together. It is many monosaccharides joined together."

·
View Answer
Biochemistry
3 months ago

Draw the Lewis structure for the polyatomic formate CHO−2 anion. Be sure to include all resonance structures that satisfy the octet rule.

·
View Answer
Biochemistry
4 months ago

Determine the molecular geometry about each interior atom and make a sketch for C2H4 (skeletal structure: H2CCH2).

·
View Answer
Biochemistry
4 months ago

The "backbone" of the DNA molecule is made up of two alternating components. What are these called?

·
View Answer
Biochemistry
4 months ago

What elements make up carbohydrates, nucleic acids, proteins, and lipids?

·
View Answer