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Electrical Engineering & Electronics /3.2: The Electron Microscope

3.2: The Electron Microscope

Electrical Engineering & Electronics12 CardsCreated 17 days ago

This deck covers key concepts related to electron microscopes, including their advantages, functions, and limitations.

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Why do electron microscopes have high resolving power?

Electron beam has a short wavelength
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Key Terms

Term
Definition
Why do electron microscopes have high resolving power?
Electron beam has a short wavelength

What are two advantages of electron microscopes?

  1. High resolving power

  2. Electrons are negatively charged and so can be focused using electromagnets

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How does a transmission electron microscope (TEM) work?
Electron gun produces a beam of electrons that is focused onto the specimen by a condenser electromagnet.
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Why do areas of the specimen appear dark?
They have absorbed electrons
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Why do areas of the specimen appear bright?
Electrons have passed through it
What is the resolving power of a TEM?
0.1nm
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3.2: The Electron Microscope
TermDefinition
Why do electron microscopes have high resolving power?
Electron beam has a short wavelength

What are two advantages of electron microscopes?

  1. High resolving power

  2. Electrons are negatively charged and so can be focused using electromagnets

How does a transmission electron microscope (TEM) work?
Electron gun produces a beam of electrons that is focused onto the specimen by a condenser electromagnet.
Why do areas of the specimen appear dark?
They have absorbed electrons
Why do areas of the specimen appear bright?
Electrons have passed through it
What is the resolving power of a TEM?
0.1nm

Why is the resolving power not always achieved in practice?

  1. Difficulties preparing the specimen limit the resolution that can be achieved

  2. A higher energy electron beam is required and this may destroy the specimen

What are the limitations of the TEM?

  1. The whole system must be in a vacuums so living specimens cannot be observed.

  2. The specimen must be very thin

  3. Complex staining process

  4. Image is in black and white

  5. The image may contain artefacts

Why do specimens have to be kept in a near vacuum to be viewed effectively?
Because electrons would be absorbed or deflected by the air if not kept in a vacuum.
How does a scanning electron microscope (SEM) WORK?
A beam of electrons is directed onto the surface of the specimen from above.
What is the resolving power of an SEM?
Around 20nm
What are the limitations of the SEM?
Same as TEM but the specimen doesn’t have to be very thin because electrons do not penetrate