Q
QuestionMicrobiology

What is the main difference between Gram-positive and Gram-negative bacteria?
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Step 2
: Understanding the Gram Staining Technique

The Gram stain is a fundamental differential staining method used to classify bacteria based on their cell wall structure. This technique, developed by Hans Christian Gram in 1884, reveals key differences in bacterial cell wall composition.

Final Answer

The main difference between Gram-positive and Gram-negative bacteria is their cell wall structure, particularly the thickness of the peptidoglycan layer and the presence or absence of an outer membrane, which determines their staining characteristics and antibiotic susceptibility.