Chemistry /Chemistry - Instrumentation in Organic Chemistry Flashcards
What is chromatography?
Chromatography is a separation technique is which a moblie phase carries a mixture of components through a stationary phase. The componests in the mixtire are selectively adbsorbed onto the stationary phase and pass through at different times.
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Term
Definition
What is chromatography?
Chromatography is a separation technique is which a moblie phase carries a mixture of components through a stationary phase. The componests in the mix...
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What is a mixture?
Substance consisting of two or more substances migled together but not chemically combined.
Name the thee types of chromotography.
Paper chromatography/ Gas chromatography/ High performance liquid chromotography (HPLC)
Describe the procedure Paper chromatography.
Using a dropper, place a drop of ink or indicator 2cm up from the bottom of the chromotography paper on a pencil line drawn. Place the chromatography ...
Describe the result Paper chromatography.
As the eluent moblie phase moves up the chromotography paper, each component colour in the ink is selectively adsorbed and appears in the chromotograp...
What is the mobile phase and the stationary phase Paper chromatography?
Moblie phase : liquid eluent/ solvent. Stationary phase: chromatography paper.
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| Term | Definition |
|---|---|
What is chromatography? | Chromatography is a separation technique is which a moblie phase carries a mixture of components through a stationary phase. The componests in the mixtire are selectively adbsorbed onto the stationary phase and pass through at different times. |
What is a mixture? | Substance consisting of two or more substances migled together but not chemically combined. |
Name the thee types of chromotography. | Paper chromatography/ Gas chromatography/ High performance liquid chromotography (HPLC) |
Describe the procedure Paper chromatography. | Using a dropper, place a drop of ink or indicator 2cm up from the bottom of the chromotography paper on a pencil line drawn. Place the chromatography paper into a beaker with 1cm of suitable eluent leave for 1 hour. |
Describe the result Paper chromatography. | As the eluent moblie phase moves up the chromotography paper, each component colour in the ink is selectively adsorbed and appears in the chromotography paper stationary at differnt times. |
What is the mobile phase and the stationary phase Paper chromatography? | Moblie phase : liquid eluent/ solvent. Stationary phase: chromatography paper. |
Name a suitable solvent for a soluable mixture and an insoluble solvent Paper chromatography. | Water (Insoluble - methanol) |
Why is the line drawn on to the chromotograpy in pencil Paper chromatography? | Avoids contamination with other types of ink. |
Where should the pencil line be drawn on the chromotography paper, explain why this is important Paper chromatography? | 2cm up the chromotopraphy paper, this avoids the solvent dissolving the ink before it moves up the paper. |
Describe the principle of atomic absorbtion spectrometry. | Different elements can be identifyed by the wavelenghts/ frequencies of light they absorb. |
Describe the principle of mass spectrometry. | Isotopes are converted into postive ions and are separated according to their mass by being accelerated by a magnetic field. |
Give three uses of a mass spectrometer. | Identifies the presence of isotopes, measures the relative abunadances of each isotope and calculate the relative atomic masses and relative molecular masses. Can detect banned drugs taken by athlete. |
Describe the principle of Infra-Red spectrometry. | Different functional groups of organic molecules absorb different frequencies of infra-red radiation and can be idetified. It can identify organic compounds. |
Name a low energy electromagnetic radiation. | Infra-red |
Name uses for IR speactrometry. | Identification of drugs and plastics, breathalyser tests. |
Describe the principle of Ultra-Violet (UV spectrometry). | The amount og ultra radiation absorbed by a substance is directly porportional to the concetration of the substance. Therefore Uv spectrometry can be used to dertime concentrations of organic compound. |
Name a high energy electrostatic radiation. | Uv radiation |
Give two uses of UV specrometry. | Test presence of drugs and measure their concentrations, Detect pigmants and measure their concentration. |