OCR A-Level Chemistry: Chapter 29 - Chromatography and Spectroscopy
This flashcard set covers the fundamental concepts of chromatography, focusing on its two main phases—stationary and mobile—as well as the materials used in Thin Layer Chromatography (TLC), such as the TLC plate. It provides quick definitions and distinctions between components.
What are the two phases for all forms of chromatography?
Stationary phase
Mobile phase
Key Terms
What are the two phases for all forms of chromatography?
Stationary phase
Mobile phase
What is the stationary phase?
Does not move
A solid or a liquid supported on a solid
What is the mobile phase?
Does move
Normally a liquid or a gas
What is a TLC plate made of?
Plastic/glass sheet
Coated with thin layer of a solid adsorbent substance such as silic...
Outline adsorption in reference to TLC
Process by which the solid silica holds the different substances in the mixture to its surface
How is the retention factor calculated?
Distance moved by the component/distance moved by the solvent front
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| Term | Definition |
|---|---|
What are the two phases for all forms of chromatography? | Stationary phase Mobile phase |
What is the stationary phase? |
A solid or a liquid supported on a solid |
What is the mobile phase? | Does move Normally a liquid or a gas |
What is a TLC plate made of? | Plastic/glass sheet Coated with thin layer of a solid adsorbent substance such as silica |
Outline adsorption in reference to TLC | Process by which the solid silica holds the different substances in the mixture to its surface |
How is the retention factor calculated? | Distance moved by the component/distance moved by the solvent front |
What is gas chromatography useful for? | Separating and identifying volatile organic compounds present in a mixture |
| Stationary - high boiling liquid adsorbed onto an inert solid support |
How does time taken to move through the column change as solubility in stationary phase increases? | Moves slower Time taken increases |
What is retention time? | Time taken for a component to travel through the column |
How is retention time used for identification? |
|
What can peak integrations for gas chromatograph be used to determine? | Concentrations of components in the sample |
What is resonance for NMR? | When the nucleus absorbs energy and rapidly flips between the two spin states |
What is the standard reference chemical for NMR? |
(CH3)4Si |
What is the purpose of D2O as a solvent in proton NMR spectroscopy? | O-H peaks no longer appear |