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Biology IB HL - 7.2 Transcription Part 1

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This flashcard deck covers key concepts of transcription in biology, including the roles of genes, promoters, RNA polymerase, and the transcription process in eukaryotes and prokaryotes.

What is a gene?

A gene is a sequence of DNA which is transcribed into RNA and contains three main parts:
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Key Terms

Term
Definition
What is a gene?
A gene is a sequence of DNA which is transcribed into RNA and contains three main parts:
What is a promoter?
The non-coding sequence responsible for the initiation of transcription
Where is the core promoted located?
The core promoter is typically located immediately upstream of the gene’s coding sequence
What is the function of the promoter?
The promoter functions as a binding site for RNA polymerase (the enzyme responsible for transcription)
What mediates the binding of RNA polymerase?
The binding of RNA polymerase to the promoter is mediated and controlled by an array of transcription factors in eukaryotes
Where do transcription factors bind?
These transcription factors bind to either proximal control elements (near the promoter) or distal control elements (at a distance)

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TermDefinition
What is a gene?
A gene is a sequence of DNA which is transcribed into RNA and contains three main parts:
What is a promoter?
The non-coding sequence responsible for the initiation of transcription
Where is the core promoted located?
The core promoter is typically located immediately upstream of the gene’s coding sequence
What is the function of the promoter?
The promoter functions as a binding site for RNA polymerase (the enzyme responsible for transcription)
What mediates the binding of RNA polymerase?
The binding of RNA polymerase to the promoter is mediated and controlled by an array of transcription factors in eukaryotes
Where do transcription factors bind?
These transcription factors bind to either proximal control elements (near the promoter) or distal control elements (at a distance)
What happens once RNA polymerase has bound to the promoter?
After RNA polymerase has bound to the promoter, it causes the DNA strands to unwind and separate
What is the coding sequence?
The region of DNA that is transcribed by RNA polymerase is called the coding sequence
When will RNA polymerase finish transcription?
RNA polymerase will continue to transcribe the DNA until it reaches a terminator sequence
Is the mechanism for transcriptional termination in eukaryotes and prokaryotes the same?
NO The mechanism for transcriptional termination differs between prokaryotes and eukaryotes
What is the antisense strand?
The antisense strand is the strand that is transcribed into RNA
What is the antisense strand’s sequence relation to the transcribed strand?
Its sequence is complementary to the RNA sequence and will be the “DNA version” of the tRNA anticodon sequence
What is another name for the antisense strand?
The antisense strand is also referred to as the template strand
What is the sense strand?
The sense strand is the strand that is not transcribed into RNA
What is the sense strand’s sequence relation to the transcribed strand?
Its sequence will be the “DNA version” of the RNA sequence (i.e. identical except for T instead of U)
What is another name for the sense strand?
The sense strand is also referred to as the coding strand (because it is a DNA copy of the RNA sequence)
Is a specific strand always anti/sense?
NO Either of the 2 polynucleotide strands may contain a gene, and hence the determination of sense and antisense is gene-specific
What is transcription?
Transcription is the process by which a DNA sequence (gene) is copied into a complementary RNA sequence by RNA polymerase
What is the first step of transcription?
Free nucleotides exist in the cell as nucleoside triphosphates (NTPs), which line up opposite their complementary base partner
How are the NTP’s joined? transcription
RNA polymerase covalently binds the NTPs together in a reaction that involves the release of the two additional phosphates
In what direction does transcription occur?
The 5’-phosphate is linked to the 3’-end of the growing mRNA strand, hence transcription occurs in a 5’ → 3’ direction
What are the 3 main steps of transcription?
The process of transcription can be divided into three main steps: initiation, elongation and termination
What occurs during initiation?
In initiation, RNA polymerase binds to the promoter and causes the unwinding and separating of the DNA strands
What occurs during elongation?
Elongation occurs as the RNA polymerase moves along the coding sequence, synthesising RNA in a 5’ → 3’ direction
What occurs during termination?
When RNA polymerase reaches the terminator, both the enzyme and nascent RNA strand detach and the DNA rewinds