How many cycles are needed to form sufficient GP's?
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| Term | Definition |
|---|---|
How many cycles are needed to form sufficient GP's? | Each GP requires one NADPH and one ATP to form a triose phosphate – so a single cycle requires six of each molecule |
How many TP molecules are used to form half a sugar molecule? | Of the six molecules of TP produced per cycle, one TP molecule may be used to form half a sugar molecule |
How many cycles are need to produce a single glucose? | Hence two cycles are required to produce a single glucose monomer, and more to produce polysaccharides like starch |
What happens to the remaining TP? | The remaining five TP molecules are recombined to regenerate stocks of RuBP (5 × 3C = 3 × 5C) |
What does the regeneration of RuBP require? | The regeneration of RuBP requires energy derived from the hydrolysis of ATP |
Who is the calvin cycle named after? | The light independent reactions are also collectively known as the Calvin cycle – named after American chemist Melvin Calvin |
What did calvin do? | Calvin mapped the complete conversion of carbon within a plant during the process of photosynthesis |
What experiment is calvin said to have carried out? | Calvin’s elucidation of photosynthetic carbon compounds is commonly classed the ‘lollipop experiment’ |
What was added to the "lollipop" apparatus? | Radioactive carbon-14 is added to a ‘lollipop’ apparatus containing green algae (Chlorella) |
What is allowed to reach the apparatus? | Light is shone on the apparatus to induce photosynthesis (which will incorporate the carbon-14 into organic compounds) |
What is done with the algae? | After different periods of time, the algae is killed by running it into a solution of heated alcohol (stops cell metabolism) |
How were the dead algae samples analysed? | Dead algal samples are analysed using 2D chromatography, which separates out the different carbon compounds |
How were the carbon samples on the chromatogram identified? | Any radioactive carbon compounds on the chromatogram were then identified using autoradiography (X-ray film exposure) |
What were the findings of the lollipop experiment? | By comparing different periods of light exposure, the order by which carbon compounds are generated was determined |
What did calvin use the findings of the lollipop experiment for? | Calvin used this information to propose a sequence of events known as the Calvin cycle (light independent reactions) |
What is the general outline of the calvin cycle? | Ribulose bisphosphate (RuBP) is carboxylated by carbon dioxide (CO2) to form a hexose biphosphate compound |
What are chloroplasts? | Chloroplasts are the ’solar energy plants’ of a cell – they convert light energy into chemical energy |
What can chloroplasts convert light energy into? | This chemical energy may be either ATP (light dependent) or organic compounds (light independent) |
What type of tissue possesses chloroplasts? | Only photosynthetic tissue possess chloroplasts (e.g. is present in leaves but not roots of plants) |
What are the 5 main features of chloroplasts? | thylakoids; grana; photosystems; stroma; lamellae |
How are thylakoids adapted to the function of the chloroplast? | flattened discs have a small internal volume to maximise hydrogen gradient upon proton accumulation |
How are grana adapted to the function of the chloroplast? | thylakoids are arranged into stacks to increase SA:Vol ratio of the thylakoid membrane |
How are photosystems adapted to the function of the chloroplast? | pigments organised into photosystems in thylakoid membrane to maximise light absorption |
How is the stroma adapted to the function of the chloroplast? | central cavity that contains appropriate enzymes and a suitable pH for the Calvin cycle to occur |
How are lamellae adapted to the function of the chloroplast? | connects and separates thylakoid stacks (grana), maximising photosynthetic efficiency |