How can a potometer be used to measure transpiration?
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| Term | Definition |
|---|---|
How can a potometer be used to measure transpiration? | When a plant is affixed to the potometer, transpiration can be indirectly identified by the movement of water towards the plant |
How can the water change in a potometer be assessed? | This water movement can be assessed as a change in meniscus level or by the movement of an air bubble towards the plant |
How can the initial starting point of the meniscus/air bubble in a potometer be altered? | The initial starting position of the meniscus or air bubble can be adjusted by introducing additional water from a reservoir |
What is important to remember when using a potometer to measure transpiration? | When measuring transpiration rates with a potometer, it is important to remember that not all water is lost to transpiration |
In what ways can water be lost apart from transpiration? | A small amount of water (~2%) is used in photosynthesis and to maintain the viable turgidity of plant cells |
What variables can affect transpiration? | temperature; humidity; light intensity; wind exposure |
In what way does temperature affect transpiration? | Increasing the ambient temperature is predicted to cause an increase in the rate of transpiration |
How does temperature change transpiration rates? | Higher temperatures lead to an increase in the rate of water vaporisation within the mesophyll, leading to more evaporation |
How can the effect of temperature variation on transpiration be tested? use of a potometer | The effect of temperature variation can be tested experimentally by using heaters or submerging in heated water baths |
In what way does humidity affect transpiration? | Increasing the humidity is predicted to cause a decrease in the rate of transpiration |
How does humidity change transpiration rates? | Humidity is the amount of water vapour in the air – less vapour will diffuse from the leaf if there is more vapour in the air |
How can the effect of humidity on transpiration be tested? use of a potometer | The effect of humidity can be tested experimentally by encasing the plant in a plastic bag with variable levels of vapour |
In what way does light intensity affect transpiration? | Increasing the light intensity to which a plant is exposed is predicted to cause an increase in the rate of transpiration |
How does light intensity change transpiration rates? | Increasing light exposure will cause more stomata to open in order to facilitate photosynthetic gas exchange |
How can the effect of light intensity on transpiration be tested? use of a potometer | The effect of light intensity can be tested experimentally by placing the plant at variable distances from a lamp |
In what way does wind exposure affect transpiration? | Increasing the level of wind exposure is predicted to cause an increase in the rate of transpiration |
How does wind exposure change transpiration rates? | Wind / air circulation will function to remove water vapour from near the leaf, effectively reducing proximal humidity |
How can the effect of wind exposure on transpiration be tested? use of a potometer | The effect of wind can be tested experimentally by using fans to circulate the air around a plant |