Laboratory 8 Muscle Soreness Lab Manual

Lab 8: Muscle Soreness & Exercise Injury – Study on delayed onset muscle soreness (DOMS), eccentric exercise effects, fatigue, and recovery using soreness ratings and performance data.

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LABORATORY8MUSCLE SORENESS ANDEXERCISE-INDUCEDMUSCLE INJURYPiirpngpLearn-Methods for measuring the time course of muscle injuryUnderstand-Mechanisms underlying muscle injury following eccentric exercise-Basic relationships between perceived exertion, fatigue, and delayed onset musclesoreness.BackgroundMost of us have experienced sore muscles following exercise or strenuous activity.Soreness that persists long after the exercise stops is called delayed onset muscle soreness(DOMS). This soreness is usually relatively mild, and goes away in several days. Alternatively,soreness can be severe enough to limit physical activity in normal subjects and reduce exerciseperformance in athletes—this soreness has been associated with muscle damage. Evidence formuscle damage includes ultrastructural disruption of myofibers seen with a microscope in biopsysamples, reduced muscle strength lasting long after muscle fatigue is thought to have ended, andthe presence of muscle specific proteins in the blood.Previous studies have shown that muscle injury is most often associated withunaccustomed exercise and with a particular kind of muscle action called an eccentric action.Muscle adapts rapidly to muscle damage such that repeating an exercise that had previouslycaused muscle soreness no longer does (called the protective effect or repeated bout effect). Theprotective effect is specific to the exercised muscle, can occur after a single bout of exercise, andcan last 3-4 weeks. The mechanisms associated with muscle resistance to injury on repeatedexposure to exercise stress are not fully understood and remains a fertile area for future research.Because DOMS is so common, and because it can disrupt exercise and function, it isimportant to develop a better understanding of what it is and what activities are associated withthe greatest muscle soreness. In this way we can find ways to avoid or minimize the negativeeffects of muscle soreness/injury.Procedures**Barring injury or sickness all students will perform the *exercise**The class should pair-up; groups of 3 are permissible if necessary. During each round of theexercise protocol the non-exercising student will supervise the exercise and record data. Thenon-exercising student is responsible for correcting their partner's stepping form.1. Prior to exercise, please record subjects' height, weight, age, and dominant leg. Musclesoreness will be recorded using a continuous scale (see data sheet below).2. Soreness in the calf muscle will be assessed using two methods beginning in lab

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