Calculating GPP and NPP

This lab explores how light affects photosynthesis by measuring dissolved oxygen in water with Elodea plants under light and dark conditions—demonstrating biochemical reactions and experimental design principles.

Daniel Miller
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Mini Lab: Calculating GPP and NPPNameOpen this Video and answer the questions while watching the video: https://youtu.be/voSQZcqgYNY1.Where and what is the sample of water used in this lab? The sample water is from an algae covered fishtank.2.What is the dissolved oxygen (DO) reading of the water sample?7.4mg/L3.How many bottles are filled?24.Why was the lid of the bottle put on under the water? All the air escapes forthe bottle to only be filled withwater and the elodea plant5.What plant is put in each bottle?The elodea plant6.Describe the treatment to each bottle. (What was done differently to each bottle?) Why?One bottle was covered and in the dark while the other was in the light to measure the differences of itscellular respiration to demonstrate the effect light has on biochemical reactions, such as photosynthesisPause the video and use what you've learned about experimental design t o answer these questions:7.List 4 constants (the things that are the same for each bottle) in this lab:Same amount of water, same plant (Elodea) source, time measured, light source.8.What is the independent variable?bottle covered or in theIight__________________________________9.What is the dependent variable? (What will we measure?)Dissolved oxygenPlay the video to get the results and answer these questions:

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10. What is t h eDOlevel of the bottle that was in t h e light?10.0nng/L11.Whatis the DO level of the bottle that wasinthe dark?5.3mg/LFill in this chart:Initial Dissolved Oxygen (ppm)(both bottles)Final Dissolved Oxygen in DarkBattle (no photosynthesis, onlyrespiration)Final Dissolved Oxygen in LightBottle (both respiration andphotosynthesis)7.4 mg/L5.3 mg/L10.0 mg/L12. Calculating Respiration Rate:Only respiration(andnot photosynthesis) can occur in a darkbottle.Respirationrate is the decrease in DO overtime. To determine this for your sample, subtract the dark DO from the initial DO,then divide it by t h e time (usually i n days). This will give!..an answer i n m g O/L/day. Use the formula below t oRespiration rate-i n i*i aL )dar(mg.) ]assist you. In a water solution, ppm = mg/Llime (days)R = (7.4-5.3)/(2)=1.05O/L/day13. Calculating Gross Primary Productivity:Gross Primary Productivity (GPP) is the total amount of carbon that wasfixed by organisms over a period of time. To determine this for your sample, subtract the dark bottle DO from thelightDOvalues,thendivideitbythe time (usuallyindays). This willgive an answer in mg O/L/day. Usethe formula below to assist you., .(light or enriched D O (mg L ) - dark D O (mgGross productivity =—- -------------------------—=------------------- ————time (days)GPP = 10.0 mg/L-5.3/2= 2.35 oyiVday14.Calculating Net Primary Productivity:Net Primary Productivity(NPP)is the totalamountof carbon that was fixedby organisms into living tissue minus that used for respiration over a period of time To determine this for yoursample, subtract the respiration rate in mg Oz/L/day from GPP in m g O/L/day. This will give an answer in mgoyL/day. Use theformula NPP = GPP - RNPP = 2.35O2/L/day-1.05Oz/L/day=1.3 Q,/L/day15. You can also use the formula below t o calculate the net primary productivity since the difference between thelight bottle DO and the initial DO is essentially the same algebraic expression as GPP minus respiration rate.
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