Kami Export - Sandra Nichols - Bio-Worksheet-Mutat
This worksheet explains DNA mutations: deletions and insertions cause frameshifts; substitutions can be silent, missense, or nonsense. It shows how mutations change amino acids and includes sickle cell anemia as an example of a missense mutation.
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NAME * " F ' ' Mutations Worksheet
Deletion. Insertion & Substitution
* inThere are several types of mutation y ..1 - — ■
DELt 1LON (a base is lost/ deleted)
INSERTION (an extra base is added/irserted)
— Deletion A insertion may cause what s called a FRAMESHIFT mutation, meaning the reading " f r a m e "
charges, thus changing the amino acid sequence from this point forward
SUBS 11 1UTION (one base is substituted for another)
— I f a substitution changes the amino acid, its called a MISSENSE mutation
— I f a substitution does not change the amino acid, its called a 5ILENT mutation
— I f a substitution changes the amino acid to a "stop," it's called a NONSENSE mutation
\ Complete the boxes below. Classify each as Deletion, Insertion or Substitution A ND os either
frameshift, missense, silent or nonsense (Hint; Deletion A Insertion will always be frameshift).
1 Original DNA Sequence: T A C
mRNA Sequence:
A C C
U G G
T T G
A A C
G C G
C G C
A C G
V G C
A C T . .
U G A
Amino Add Sequence: Met Trp Mn M Cjffl Slop
Mutated DNA Sequence =1 T A C
Whafsthe mRNA sequence? u ft
A T C
u Q 5
T T G
* A C
G C G
C <5 C
A C G
U G C
AC T ...
U ft A (Circle the change)
amino add sequence? f.,1ethi Oil i ne-{Stpp)
Will chert likely be effects? ■■■■< What type of mutation is this? Subs; till tuition Ncf'is nsf-
Mutated DNA Sequence #2 T A C G A C C T TiG G C G A C G A C T ...
Whafsthe mRNA sequence? A U G C)UG 3 A A C C G C U G C U G A (Circle the change)
amino add sequence? Mel Set C , u Pro Leu Pro
Will there likely he effects? Yes What type of mutation 15 tiu5? |T seMiori Frame Shifl----------------------------------
Mutated DNA Sequence #3 T A C A C C T T A G C G A C G1A C T...
Whafsthe mRNA sequence? AUG U G G A A ® C G C U G C U G A (Circle the change)
amino add sequence? Trp A«n Str Cy« Slap
Will there likely be effects? What type of mutation is this? Sctshtutisi- i lent V_tat.oi. __________
Mutated DNA Sequence £4 T A C A C C T T H T G C G A C T A C T...
Whafs the mRNA sequence? A U G U G G A A C C G C U G U G A (Circle the change)
amino add sequence? Mel Trp Ash Arg Stop Stop
Will there likely be effects? yes — hat type of mutation is this? s;.a-<,nuiinii h ________________
Deletion. Insertion & Substitution
* inThere are several types of mutation y ..1 - — ■
DELt 1LON (a base is lost/ deleted)
INSERTION (an extra base is added/irserted)
— Deletion A insertion may cause what s called a FRAMESHIFT mutation, meaning the reading " f r a m e "
charges, thus changing the amino acid sequence from this point forward
SUBS 11 1UTION (one base is substituted for another)
— I f a substitution changes the amino acid, its called a MISSENSE mutation
— I f a substitution does not change the amino acid, its called a 5ILENT mutation
— I f a substitution changes the amino acid to a "stop," it's called a NONSENSE mutation
\ Complete the boxes below. Classify each as Deletion, Insertion or Substitution A ND os either
frameshift, missense, silent or nonsense (Hint; Deletion A Insertion will always be frameshift).
1 Original DNA Sequence: T A C
mRNA Sequence:
A C C
U G G
T T G
A A C
G C G
C G C
A C G
V G C
A C T . .
U G A
Amino Add Sequence: Met Trp Mn M Cjffl Slop
Mutated DNA Sequence =1 T A C
Whafsthe mRNA sequence? u ft
A T C
u Q 5
T T G
* A C
G C G
C <5 C
A C G
U G C
AC T ...
U ft A (Circle the change)
amino add sequence? f.,1ethi Oil i ne-{Stpp)
Will chert likely be effects? ■■■■< What type of mutation is this? Subs; till tuition Ncf'is nsf-
Mutated DNA Sequence #2 T A C G A C C T TiG G C G A C G A C T ...
Whafsthe mRNA sequence? A U G C)UG 3 A A C C G C U G C U G A (Circle the change)
amino add sequence? Mel Set C , u Pro Leu Pro
Will there likely he effects? Yes What type of mutation 15 tiu5? |T seMiori Frame Shifl----------------------------------
Mutated DNA Sequence #3 T A C A C C T T A G C G A C G1A C T...
Whafsthe mRNA sequence? AUG U G G A A ® C G C U G C U G A (Circle the change)
amino add sequence? Trp A«n Str Cy« Slap
Will there likely be effects? What type of mutation is this? Sctshtutisi- i lent V_tat.oi. __________
Mutated DNA Sequence £4 T A C A C C T T H T G C G A C T A C T...
Whafs the mRNA sequence? A U G U G G A A C C G C U G U G A (Circle the change)
amino add sequence? Mel Trp Ash Arg Stop Stop
Will there likely be effects? yes — hat type of mutation is this? s;.a-<,nuiinii h ________________
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Subject
Biology