Back to AI Flashcard MakerAnatomy and Physiology /Histology - Intro to Histology
Histology - Intro to Histology
This deck covers key concepts in histology, focusing on microscopy techniques, staining methods, and cellular structures.
focused with optincal lens (max resoving poswr ~0.2 microns).
Light microscopy (LM)
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Key Terms
Term
Definition
focused with optincal lens (max resoving poswr ~0.2 microns).
Light microscopy (LM)
Electrons interact with tissue components (B&W images). Focused with electromagnetic lens (resolution 1000x LM)
Electron microscopy (EM)
Shows internal structure
Transmission (TEM)
Shows external (surface) structure
Scanning (SEM)
splits cell membranes (p-face, e-face)
TEM freeze fracture technique
Interact with acids (basophilic structures). Stain acidic components blue or purple. E.g. nucleic acids (RNA< DNA, RER), glycosaminoclycans (GAGs)
Base-like stains
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| Term | Definition |
|---|---|
focused with optincal lens (max resoving poswr ~0.2 microns). | Light microscopy (LM) |
Electrons interact with tissue components (B&W images). Focused with electromagnetic lens (resolution 1000x LM) | Electron microscopy (EM) |
Shows internal structure | Transmission (TEM) |
Shows external (surface) structure | Scanning (SEM) |
splits cell membranes (p-face, e-face) | TEM freeze fracture technique |
Interact with acids (basophilic structures). Stain acidic components blue or purple. E.g. nucleic acids (RNA< DNA, RER), glycosaminoclycans (GAGs) | Base-like stains |
Interact with bases (acidophilic structures). Stain basic components pink, red, or orange. E.g. mitochondria, collagen | Acid-like stains |
acid-like stain (reddish) | eosin |
base-like stain (blue) | hematoxylin |
Stains carbohydrates magenta. E.g., glycoproteins in mucin | Periodic-Acid Schiff (PAS) |
coffee bean staining pattern of nucleus | Brenner tumor |
Stains collagen fibers blue or green | Trichrome |
Stains reticular fibers black | Silver |
Stains elastic fibers reddish-brown or black | Orcein |
stains lipids red | Sudan Red |
stains lipids black | Osmic acid |
Look at nuclei (tend to be similar in size among cells) | How to see scale |
biological yardstick (~8 microns) | Red blood cell (RBS) |
tissue shrinkage & lost molecules, “cracks” & “spaces” | Artifacts |